產品特點:
Sphinx 擴展您檢測的選擇性 ※ 使用C18和“苯基”雜和鍵合，擴展色譜柱的選擇性分離
※ PH穩定性好 1—10之間穩定使用
※ 多步高密度鍵合技術，保證樣品出峰尖銳對稱
※ 低流失，滿足液相質譜LC-MS使用要求
為避免常規高密度鍵合C18色譜柱的疏水性過強對極性化合物分析能力差的問題，Sphinx選擇性鍵入“苯基”官能團，增加了和帶雙鍵樣品間的∏-∏作用，具有特殊的選擇性能，所以sphinx特別適合分析帶“苯環”和“雙鍵”的樣品。
High purity silica phase for HPLC
Bifunctional RP phase, balanced ratio of propylphenyl and C18 ligands
endcapped · 15 % C · USP L1 / L11
Key features:
Distinct selectivity based on well-balanced bifunctional surface coverage Widens the scope for method development based on additional π-π interactions Suitable for LC/MS due to low bleeding characteristics |
Technical characteristics:
Octadecyl and propylphenyl modified silica
pore size 110 Å; particle sizes 1.8 μm, 3 μm and 5 μm
carbon content 15 %; pH stability 1–10;
high reproducibility |
Recommended application:
Quinolone antibiotics, sulfonamides, xanthines, substituted aromatics
For applications click here.產品應用: 下圖表明Sphinx和常規C8/C18色譜柱在鍵合方法和選擇性上的區別 流動相：甲醇：水=60:40流 速：1.0毫升/分鐘
進樣量：3 ul溫 度：30℃檢測器：UV 270nm
樣 品：1.兒茶素 2.蘆丁 R1=R3=DH,R2=O-rutinose 3.漆黃素 R1=R3=OH,R2=H 4.槲皮素 R1=R2=R3=OH 5.酚R1=H,R2=R3=OH 6.異鼠李素 R1=OCH3，R2=R3=OH 訂貨信息: EC standard columns for analytical HPLC | | Ordering information | Eluent in column acetonitrile – water |
Length -> | 30 mm | 50 mm | 75 mm | 100 mm | 125 mm | 150 mm | 250 mm | NUCLEODUR® Sphinx RP, 1.8 μm | 2 mm ID | 760821.20 | 760822.20 | 760825.20 | 760823.20 | | 760824.20 | | 3 mm ID | 760821.30 | 760822.30 | | 760823.30 | | | | 4 mm ID | 760821.40 | 760822.40 | | 760823.40 | | | | 4.6 mm ID | 760821.46 | 760822.46 | | 760823.46 | | | | EC guard columns*: | 4 x 2 mm | 761920.20 | | 4 x 3 mm | 761920.30 | | NUCLEODUR® Sphinx RP, 3 μm | 2 mm ID | | 760806.20 | | 760812.20 | 760807.20 | 760805.20 | 760808.20 | 3 mm ID | | 760806.30 | | 760812.30 | 760807.30 | 760805.30 | 760808.30 | 4 mm ID | | 760806.40 | | 760812.40 | 760807.40 | 760805.40 | 760808.40 | 4.6 mm ID | | 760806.46 | 760813.46 | 760812.46 | 760807.46 | 760805.46 | 760808.46 | EC guard columns*: | 4 x 2 mm | 761921.20 | | 4 x 3 mm | 761921.30 | | CC guard columns**: | 8 x 3 mm | 761557.30 | | 8 x 4 mm | 761557.40 | | NUCLEODUR® Sphinx RP, 5 μm | 2 mm ID | | 760800.20 | | 760809.20 | 760801.20 | 760802.20 | 760803.20 | 3 mm ID | | 760800.30 | | 760809.30 | 760801.30 | 760802.30 | 760803.30 | 4 mm ID | | 760800.40 | | 760809.40 | 760801.40 | 760802.40 | 760803.40 | 4.6 mm ID | | 760800.46 | 760815.46 | 760809.46 | 760801.46 | 760802.46 | 760803.46 | EC guard columns*: | 4 x 2 mm | 761922.20 | | 4 x 3 mm | 761922.30 | | CC guard columns**: | 8 x 3 mm | 761550.30 | | 8 x 4 mm | 761550.40 | | Guard columns in packs of 3, EC columns in packs of 1 | Guard columns for EC columns | | EC columns with ID | Use guard
column holder | | | | 2 mm | 3 mm | 4 mm | 4.6 mm | * | Column Protection System | EC guard column | 4/2 | 4/3 | 4/3 | 4/3 | REF 718966 | ** | ChromCart® guard column system | CC guard column | 8/3 | 8/3 | 8/4 | 8/4 | REF 721359 | Custom-packed EC columns with other dimensions are available on request. | top of page |
VarioPrep columns for preparative HPLC |
| Ordering information | Eluent in column acetonitrile – water |
Length | 10 mm ID | 21 mm ID | 32 mm ID | 40 mm ID | NUCLEODUR® Sphinx RP, 5 μm | 50 mm | 762372.100 | 762372.210 | | | 125 mm | 762375.100 | 762375.210 | | | 150 mm | | | | 762371.400 | 250 mm | 762373.100 | 762373.210 | 762373.320 | 762373.400 | VP guard columns with NUCLEODUR® Sphinx RP | | 8 mm ID | 16 mm ID | 32 mm ID | 10 mm* | 762390.80 | 762390.160 | | | 15 mm* | | | 762392.320 |
* VP guard column | Size | Pack of | for VP column | required holder | REF | VP 10/8 | 10 x 8 mm ID | 2 | 10 mm ID | 8 mm | 718251 | VP 10/16 | 10 x 16 mm ID | 2 | 21 mm ID | 16 mm | 718256 | VP 15/32 | 15 x 32 mm ID | 1 | 32–40 mm ID | 32 mm | 718253 | Custom-packed VP columns with other dimensions are available on request. | For details of the VarioPrep column system click here. |
Standard program* of columns packed with NUCLEODUR® Sphinx RP |
| Column system | Particle size: | 1.8 μm | 3 μm | 5 μm | Analytical | EC columns (standard system, 2–4.6 mm) |
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| Preparative | VarioPrep columns (VP, ≥ 8 mm) |
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| if you do not know our HPLC column systems, click the "i" for further information | * Please note! Most combinations of MN column types, available column dimensions and particle sizes can be custom-packed on request. |
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