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葡萄糖酸銅 號(hào)：527-09-3  
英文名稱：Copper gluconate；Copper(II) gluconate；D-Gluconic acid copper(II) salt   
其他名稱：雙(D-葡糖酸)銅；葡萄糖酸銅(II)    
號(hào)：527-09-3或13005-35-1   
C12H22CuO14=453.84   
級(jí)別：BR   
純度：≥98.0%   
干燥失重：≤2.0%   
性狀(以下信息僅供參考)：白色或淺藍(lán)色至藍(lán)綠色結(jié)晶或粉末，無臭，極易溶于水，難溶于乙醇   
用途：本品僅供科研，不得用于其它用途。   
保存：RT 葡萄糖酸銅 號(hào)：527-09-3儲(chǔ)存條件：
避光、干燥陰涼處封閉貯存,嚴(yán)禁與有毒、有害物品混放、混運(yùn)。本品為非危險(xiǎn) 產(chǎn)品可按一般化學(xué)品運(yùn)輸,輕搬動(dòng)輕放,防止日曬、雨淋！受熱、受潮、受光后易喪失活力,保存期短,因此貯存和運(yùn)輸條件比較苛刻。
運(yùn)輸：汽車運(yùn)輸、EMS郵政快遞，申通快遞等， 款到上海3天內(nèi)發(fā)貨；
售后：如您對(duì)我們的產(chǎn)品服務(wù)及技術(shù)指標(biāo)有特殊要求，請(qǐng)及時(shí)通知我方。
存儲(chǔ)：應(yīng)貯存在干燥清潔避光的環(huán)境中，嚴(yán)禁與有毒物質(zhì)混放，以免污染（保質(zhì)期為兩年）。
葡萄糖酸銅 號(hào)：527-09-3主要優(yōu)級(jí)純、分級(jí)純和化學(xué)純3種：
（1）優(yōu)級(jí)純（GR：Guaranteed reagent），又稱一級(jí)品或保證試劑，99.8%，這種試劑純度Z高，雜質(zhì)含量Z低，適合于重要精密的分析工作和科學(xué)研究工作，使用綠色瓶簽。
（2）分析純（AR），又稱二級(jí)試劑，純度很高，99.7%，略次于優(yōu)級(jí)純，適合于重要分析及一般研究工作，使用紅色瓶簽。
（3）化學(xué)純（CP），又稱三級(jí)試劑，≥ 99.5%，純度與分析純相差較大，適用于工礦、學(xué)校一般分析工作。使用藍(lán)色（深藍(lán)色）標(biāo)簽。
（4）實(shí)驗(yàn)試劑（LR：Laboratory reagent），又稱四級(jí)試劑。
保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品介紹 This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants.
Function : SirtT1 75 kDa fragment: catalytically inactive 75SirT1 may be involved in regulation of apoptosis. May be involved in protecting chondrocytes from apoptotic death by associating with cytochrome C and interfering with apoptosome assembly.
Subunit : Found in a complex with PCAF and MYOD1 (By similarity). Component of the eNoSC complex, composed of SIRT1, SUV39H1 and RRP8. Interacts with HES1, HEY2 and PML. Interacts with RPS19BP1/AROS. Interacts with KIAA1967/DBC1 (via N-terminus); the interaction disrupts the interaction between SIRT1 and p53/TP53. Interacts with SETD7; the interaction induces the dissociation of SIRT1 from p53/TP53 and increases p53/TP53 activity. Interacts with MYCN, NR1I2, CREBZF, TSC2, TLE1, FOS, JUN, NR0B2, PPARG, NCOR, IRS1, IRS2 and NMNAT1. Interacts with HNF1A; the interaction occurs under nutrient restriction. Interacts with SUZ12; the interaction mediates the association with the PRC4 histone methylation complex which is specific as an association with PCR2 and PCR3 complex variants is not found. Interacts with -1 tat.
Subcellular Location : Nucleus, PML body. Cytoplasm. Note=Recruited to the nuclear bodies via its interaction with PML. Colocalized with APEX1 in the nucleus. May be found in nucleolus, nuclear euchromatin, heterochromatin and inner membrane. Shuttles between nucleus and cytoplasm.
SirtT1 75 kDa fragment: Cytoplasm. Mitochondrion.
Tissue Specificity : Widely expressed.
Post-translational modifications : Methylated on multiple lysine residues; methylation is enhanced after DNA damage and is dispensable for deacetylase activity toward p53/TP53.
Phosphorylated. Phosphorylated by STK4/MST1, resulting in inhibition of SIRT1-mediated p53/TP53 deacetylation. Phosphorylation by MAPK8/JNK1 at Ser-27, Ser-47, and Thr-530 leads to increased nuclear localization and enzymatic activity. Phosphorylation at Thr-530 by DYRK1A and DYRK3 acivates 葡萄糖酸銅 號(hào)：527-09-3deacetylase activity and promotes cell survival. Phosphorylation by mammalian target of rapamycin complex 1 (mTORC1) at Ser-47 inhibits deacetylation activity. Phosphorylated by CaMK2, leading to increased p53/TP53 and NF-kappa-B p65/RELA deacetylation activity (By similarity). Phosphorylation at Ser-27 implicating MAPK9 is linked to protein stability. There is some ambiguity for some phosphosites: Ser-159/Ser-162 and Thr-544/Ser-545.
Proteolytically cleaved by cathepsin B upon TNF-alpha treatment to yield catalytic inactive but stable SirtT1 75 kDa fragment (75SirT1).
S-nitrosylated by GAPDH, leading to inhibit the NAD-dependent protein deacetylase activity (By similarity).
Similarity : Belongs to the sirtuin family.
Contains 1 deacetylase sirtuin-type domain.
Database links : UniProtKB/Swiss-Prot: Q96EB6.2
在Sirtuin蛋白家族中，sirtuin 1參與多種新陳代謝活動(dòng)，包括DNA的自我保護(hù)和修復(fù)，抑制脂質(zhì)過氧化積累，抑制其他細(xì)胞凋亡相關(guān)基因的表達(dá)以及和細(xì)胞壽命相關(guān)的活動(dòng)。限制攝入的熱量可以加強(qiáng)SIRT1的表達(dá)，從而延長(zhǎng)了壽命。
英文名稱  Anti-Smac/DIABLO
中文名稱  線粒體促凋亡蛋白抗體
別    名  0610041G12Rik; DBLOH_HUMAN; DBOH; DBOH; diablo; Diablo homolog (Drosophila); Diablo homolog (Drosophila); Diablo homolog; Diablo homolog mitochondrial; DIABLO S; DIABLO S; Direct IAP binding protein with low pI; Direct IAP binding protein with low pI; Direct IAP-binding protein with low pI; FLJ10537; FLJ25049; mitochondrial; Mitochondrial Smac protein; Second mitochondria derived activator of caspase; Second mitochondria derived activator of caspase; Second mitochondria-derived activator of caspase; SMAC 3; Smac; Smac protein; Smac protein; SMAC3; SMAC3; 
濃    度  1mg/1ml
規(guī) 格  0.1ml/100μg  0.2ml/200μg
抗體來源  Rabbit 
克隆類型  polyclonal
交叉反應(yīng)  Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse
產(chǎn)品類型  一抗   
研究領(lǐng)域  腫瘤 細(xì)胞生物 細(xì)胞凋亡 新陳代謝 線粒體 
蛋白分子量  predicted molecular weight: 21kDa
性    狀  Lyophilized or Liquid
免 疫 原  KLH conjugated synthetic peptide derived from human Smac C-terminus
亞    型  IgG
純化方法  affinity purified by Protein A
儲(chǔ) 存 液  0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
產(chǎn)品應(yīng)用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500
（石蠟切片需做抗原修復(fù)）
 not yet tested in other applications.
 optimal dilutions/concentrations should be determined by the end user. 
保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品介紹 This gene encodes an inhibitor of apoptosis protein (IAP)-binding protein. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and it moderates the caspase inhibition of IAPs. Multiple polyadenylation sites have been found for this gene. Several alternatively spliced transcript variants that encode distinct isoforms have been described for this gene but the validity of some transcripts, and their predicted ORFs, has not been determined conclusively. The inhibitor of apoptosis (IAP) proteins regulate programmed cell death by inhibiting members of the caspase family of enzymes. A novel mammalian protein that binds to IAPs and neutralizes their inhibitory effect on caspases has been designated Smac/DIABLO. This is a mitochondrial protein that is released along with cytochrome c during apoptosis and activates the cytochrome c/Apaf-1/caspase-9 pathway. Analysis of the structural basis of Smac/DIABLO reveals that the N-terminal amino acids are required for binding of Smac/DIABLO to IAPs and activation of caspases. Smac/DIABLO is expressed in a variety of human and mouse tissues.
Function : Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). Inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases. Isoform 3 attenuates the stability and apoptosis-inhibiting activity of XIAP/BIRC4 by promoting XIAP/BIRC4 ubiquitination and degradation through the ubiquitin-proteasome pathway. Isoform 3 also disrupts XIAP/BIRC4 interacting with processed caspase-9 and promotes caspase-3 activation. Isoform 1 is defective in the capacity to down-regulate the XIAP/BIRC4 abundance.
Subunit : Homodimer. Interacts with NGFRAP1/BEX3 (By similarity). Interacts with BIRC2/c-IAP1, BIRC3/c-IAP2, XIAP/BIRC4, BIRC6/bruce and BIRC7/livin. Interacts with the monomeric and dimeric form of BIRC5/survivin.
Subcellular Location : Mitochondrion. Note=Released into the cytosol when cells undergo apoptosis.
Tissue Specificity : Ubiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes. Isoform 3 is ubiquitously expressed.
Post-translational modifications : Ubiquitinated by BIRC7/livin.
DISEASE : Deafness, autosomal dominant, 64 (DFNA64) [MIM:614152]: A form of non-syndromic sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. Note=The disease is caused by mutations affecting the gene represented in this entry.
Database links :
UniProtKB/Swiss-Prot: Q9NR28.1
Entrez Gene: 56616 Human
Entrez Gene: 66593 Mouse
Entrez Gene: 288753 Rat
Omim: 605219 Human
SwissProt: Q9NR28 Human
SwissProt: Q9JIQ3 Mouse
Unigene: 169611 Human
Unigene: 46716 Mouse
Smac（second mitochondria-derived activator of caspase）又稱DIABLO（direct I